overview amino acid analyzer ion chromatography hpcpc applications hpcpc hpcpc accessories various detectors column ovens, degassers off. sample preparation on. sample preparation auto. sample preparation valves column selector online injector & sampler centrion ws autosampler encon p & t concentrator markelov autosampler est cobra autosampler hplc pumps syringe pumps flavourspec breathspec bp-ims gc-ims analytical ims uv-ims µims-odour ims-odour
 
 
 
 
HPCPC APPLICATIONS         CHROMATOGRAPHY SYSTEM
 
 
HIGH PERFORMANCE CENTRIFUGAL PARTITION CHROMATOGRAPHY
 
High Performance Centrifugal Partition Chromatography, HPCPC, is a new technique of Liquid Chromatography, which is support free and which finds its place among the several liquid chromatographic methods as shown on the following chart:
 
 
There are four main advantages of the HPCPC as follows:
 
1) No loss of sample: Both mobile and stationary phases are liquids and can be collected for total recovery.
2) The volume ratio of stationary to mobile phase is definitely much higher, which leads to higher capacities and better resolution with no requirement of a high number of theoretical plates.
3) The extreme flexibility of biphasic system: The mixtures of the two, three or four solvents, which allows to modify the selectivity of a system in order to get a pure compound in the HPCPC polarities of both phases can be smoothly modified.
4) The reduced solvent consumption is ten times less than preparative scale chromatography for the same throughput, it should be environmentally considered. Separation accomplished with laboratory HPCPC can be directly scaled up to production scale HPCPC.
 
Without a solid stationary phase support, The HPCPC system effectively separate, isolate and purify milligrams to multigrams.
APPLICATIONS

Bio Polymers

Rare Metal and Earth

Beta-Carotene Metal Ions
Bioreactor Modified Oligo-Peptides
Chlorophyll Monosac Charide
Chromomycine N-propyl-p-hydroxybenzoate & Prednisolone
Diterpene Esters from Daphne extract Cavanocobalamin
Egg Yolk Lecithin L-Ascorbic Acid
Enzymes from Yeast extract Fatty Acid Esters

Fatty Acids 1 Saturated Fatty Acids (Stearic Acid, Palmitic Acid, Myristic Acid and Lauric Acid)

Fatty Acids 2 Unsaturated Fatty Acids (Stearic Acid, Linolenic Acid and Linoleic Acid)
Fish Oil Hydrolysate
Fungous Toxin Nivalenol
Herb Medicine (Isolation of Faicalin from Scutellariae Radix)
Herb Medicine (Isolation of Glycyrrhizin from Glycyrrhiza Galbra)
Herb Medicine (Isolation of Secologanin from Symphoricarpos Albus)
Herb Medicine (Oridonin and Enmein)
Hydrophobicity Parameters of Drugs Indole Acetic Acids
L-Leucine Dehydrogenase Lipid A from Cell Membrane of Salmonella
Nucleoside (Adenine, 2-Deoxyadenine, 2-Deoxycytidine, 2-Deoxyguanosine and Cytidine)
Oligo-Saccharides Olive Oil with Lipase
Paeoniflorin from Peony extract Paeoniflorin from Peony extract (Large scale)
Phthalic Acid Isomers from Dilute Aqueous Solution Pigments from Gardenia
Pigments in Sea Squirt Proteins in Aqueous two phase system 1
Proteins in Aqueous two phase system 2
Quinones (Anthraquinone, p-Benzoquione and Alpha-Naphthoquione)
Saiko Sapoins 1 Saiko Sapoins 2
Salmon Sperm DNA Saponins from Ginseng
Serum Proteins Synthetic Lipid A
Tannins Terpendoids in Tobacco Leaves
Tocopherols Tocopherols (Large scale)
Toxic Substance in Azalea Ubiquinone
Vegetable Oil Hydrolysate
Vitamins (A, D and E) Vitamins (Nicotinamide)
Riboflavine Thiamine

Fermentation Products

Amino Acids

Fine Chemicals

Alkaloids

Foods and Additives

Co-enzymes

Genetically Engineered Substances

Fatty Acids

Natural Organic Compounds

Iridoid Glucosides

Petrochemicals

Natural Products

Physiological Activated Substances

Peptides

Pharmaceuticals Phospholipids
Proteins Steroidal Saponins
 
HPCPC Principle:
 
The HPCPC is a new liquid chromatographic technique that utilises liquid-liquid partition, counter current distribution in the absence of a solid support to perform separations of complex mixtures of chemical substances.
The HPCPC is an alternative to packed-bed columns for preparative HPCPC and operates by classical liquid-liquid partitioning in a high performance centrifugal system.
A solid stationary phase is not used. Instead, stationary phase liquid is retained by centrifugal force in discrete partition channels within a unique patented circular partition disk pack.
A packed column generally contains only 2 to 7 percent of stationary phase, severely limits its capacity.
In an HPCPC system, the column contains between 50 and 80 percent stationary phase.
The stationary phase is held in numerous discrete partition cells. Micro-droplets of mobile phase liquid pass continuously through the stationary phase liquid. Any two-phase solvent mixture can be used at any pH to perform normal and reversed phase chromatographic separations.
An injected sample, carried by the mobile phase, moves sequentially through the partition channels, where components are partitioned between the mobile and stationary liquid phase, separated from each other on the basis of differences in their partition coefficients and eluted.
 
 
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